feng zhang lab (Addgene inc)

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Addgene inc feng zhang lab
Feng Zhang Lab, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 2950 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/feng+zhang+lab/pSpCas9(BB)-2A-Puro+(PX459)+V2%2E0+(Plasmid+%2362988)/pm41780531-231-29-32
Average 96 stars, based on 2950 article reviews

feng zhang lab - by Bioz Stars, 2026-10

96/100 stars

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CRISPR:

Article Title: The Vps13-like protein BLTP2 regulates phosphatidylethanolamine levels to maintain plasma membrane fluidity and breast cancer aggressiveness.
Article Snippet: .. Generation of CRISPR KO cell lines BLTP2 was knocked out in HeLa cells using the pSpCas9(BB)-2A-Puro plasmid54 and sgRNA targeting Exon2. sgRNA was ordered as oligonucleotides (sgBLTP2-F and sgBLTP2-R) and cloned using a BbsI restriction enzyme digested vector according to the method described by the Feng Zhang lab at the MIT in Addgene (Addgene, 62988)54. .. Generation of CRISPR KO cell lines BLTP2 was knocked out in HeLa cells using the pSpCas9(BB)-2A-Puro plasmid54 and sgRNA targeting Exon2. sgRNA was ordered as oligonucleotides (sgBLTP2-F and sgBLTP2-R) and cloned using a BbsI restriction enzyme digested vector according to the method described by the Feng Zhang lab at the MIT in Addgene (Addgene, 62988)54.

Article Title: Sustained poly(ADP-ribosyl)ation limits RPA loading and attenuates ATR-CHK1 signaling upon replication fork collapse
Article Snippet: .. We used a modified version of the CRISPR-Cas9 called D10A or nickase, pSpCas9n(BB)-2A-Puro (PX462) V2.0, which was a kind gift from Feng Zhang lab (Addgene, plasmid #62987) ( Ran et al , 2013 ). .. The 4 guide-RNAs (gRNA) were cloned into pUC-H1-gRNA, a kind gift from Shenglin Huang lab (Addgene, plasmid #61089) ( Zheng et al , 2014 ).

Clone Assay:

Article Title: The Vps13-like protein BLTP2 regulates phosphatidylethanolamine levels to maintain plasma membrane fluidity and breast cancer aggressiveness
Article Snippet: .. BLTP2 was knocked out in HeLa cells using the pSpCas9(BB)-2A-Puro plasmid and sgRNA targeting Exon2. sgRNA was ordered as oligonucleotides (sgBLTP2-F and sgBLTP2-R) and cloned using a BbsI restriction enzyme digested vector according to the method described by the Feng Zhang lab at the MIT in Addgene (Addgene, 62988) . ..

other:

Article Title: Global profiling of nascent chain interactors reveals TRIM25 as a co-translational E3 ubiquitin ligase.
Article Snippet: Short article Global profiling of nascent chain interactors reveals TRIM25 as a co-translational E3 ubiquitin ligase

Article Title: Cross-species tropism of AAV.CPP.16 in the respiratory tract and its gene therapies against pulmonary fibrosis and viral infection.
Article Snippet: Human embryonic kidney (HEK293T) cells (CRL-3216) and Human nasal epithelial cell RPMI 2650 (CCL-30) were purchased from the American Type Culture Collection (ATCC).

Modification:

Plasmid Preparation:

Article Title: Rainbow Nucleus Charts Dynamic Interactome of Membrane-less Organelles
Article Snippet: .. The pSpCas9(BB)-2A-GFP (PX458) was a gift from Feng Zhang Lab (Addgene plasmid # 48138; http://n2t.net/addgene:48138 ; RRID:Addgene_48138). .. Briefly, complementary guide oligonucleotides containing overhangs for pSpCas9(BB) BbsI sites were annealed and phosphorylated in a reaction containing 1 μL 100 μM forward oligo, 1 μL 100 μM reverse oligo, 1 μL 10x T4 ligation buffer (B0202S, NEB), 1 μL T4 polynucleotide kinase (B0201S, NEB), and 6 μL nuclease-free water.

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